External quality assessment of flow cytometric HLA-B27 typing
نویسندگان
چکیده
منابع مشابه
HLA-B27 typing by use of flow cytofluorometry.
We describe the use of flow cytofluorometry to type lymphocytes for HLA-B27 antigens. We modified the cytotoxicity test of Terasaki and McClelland [Nature (London) 1964;204:998] for use with a flow cytofluorometer. In a second assay we used a commercially available monoclonal anti-HLA-B27 antibody and indirect immunofluorescence staining of cell surfaces. Although this antibody cross reacts wit...
متن کاملHLA-B27 typing in the categorisation of uveitis in a HLA-B27 rich population.
AIMS To determine whether HLA-B27 typing helps the clinician in the diagnostic examination of uveitis in a HLA-B27 rich population and also whether the clinical picture of HLA-B27 positive unilateral acute or recurrent anterior uveitis (AAU) is distinguishable from the idiopathic negative form. METHODS During a 3 year period 220 consecutive patients with undetermined uveitis at onset were exa...
متن کاملFlow Cytometric Human Leukocyte Antigen-B27 Typing with Stored Samples for Batch Testing
BACKGROUND Flow cytometry (FC) HLA-B27 typing is still used extensively for the diagnosis of spondyloarthropathies. If patient blood samples are stored for a prolonged duration, this testing can be performed in a batch manner, and in-house cellular controls could easily be procured. In this study, we investigated various methods of storing patient blood samples. METHODS WE COMPARED FOUR STORA...
متن کاملLong-term stabilized blood samples as controls for flow cytometric HLA-B27 screening: a feasibility study.
BACKGROUND Long-term stabilized blood samples are potentially useful as positive or negative procedure controls for flow cytometric HLA-B27 screening, and could serve as test samples in an external quality assessment (EQA) scheme. We evaluated long-term stabilized whole blood specimens as prepared for the UK NEQAS for Leucocyte Immunophenotyping EQA scheme (Sheffield, UK). METHODS Peripheral ...
متن کاملThe flow cytometric labeling pattern in HLA-B27 detection may suggest cross reactivities
Background: The detection of HLA-B27 expression is one of the most powerful diagnostic tools in ankylosing spondylitis and other spondylarthropaties, hence the importance of a reliable and accurate detection method. Flow cytometry is often used as an alternative HLA-B27 typing technique, mainly because it is a rapid test. However, even though it is based on various monoclonal antibodies, their ...
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ژورنال
عنوان ژورنال: Cytometry
سال: 2000
ISSN: 0196-4763,1097-0320
DOI: 10.1002/(sici)1097-0320(20000415)42:2<95::aid-cyto2>3.0.co;2-8